To immunoprecipitate mutant protein, the cnd2-1 gene was tagged at the carboxy terminus with eight times Myc or jellyfish green fluorescent protein (GFP), and chromosomally integrated. Using anti-Myc ...
The Anaphase Promoting Complex/Cyclosome (APC/C), a large ubiquitin ligase, targets key cell-cycle regulators such as cyclin A2 (the major A-type cyclin in human somatic cells) and cyclin B1 for ...